| Ukrainian Community in Barrie, Online/Web Service | Barrie |
| Ukrainian National Federation of Canada, Barrie Simcoe, UNF Barrie Simcoe | Barrie |
| Ukuladies | Barrie |
| U-Links Centre for Community-Based Research | Minden Hills |
| Uneda Cab | Cramahe |
| Unique Support Services | Port Elgin |
| United Association for Plumbers, Steamfitters and Welders, Barrie Office, Local 46 | Barrie |
| United Church of Canada, Emmanuel United Church | Peterborough |
| United Church of Canada, Keene United Church | Otonabee-South Monaghan |
| United Church of Canada, Lakefield United Church | Lakefield |
| United Church of Canada, Norwood United Church | Asphodel-Norwood |
| United Church of Canada - Simcoe Presbytery, Penetanguishene, Camp Simpresca | Penetanguishene |
| United Senior Citizens of Ontario, Zone 42 - Peterborough Branch | Peterborough |
| United Way for the City of Kawartha Lakes (Haliburton) | Kawartha Lakes |
| United Way of Bruce Grey, Julie McArthur Regional Recreation Centre - Ice Pads, Puck Pigs Hockey Program | Owen Sound |
| United Way of Bruce Grey, Backpack Program - 211 I&R Procedures and Information | Owen Sound |
| United Way of Bruce Grey, Backpack Program | Owen Sound |
| United Way of Bruce Grey, Financial Literacy | Owen Sound |
| United Way of Bruce Grey | Owen Sound |
| United Way of Bruce Grey, Utility Assistance Program | Owen Sound |
| United Way Perth-Huron, No Physical Address - Huron County, Urgent Needs Fund | South Huron |
| United Way Perth-Huron, No Physical Address - Perth County, Urgent Needs Fund | Stratford |
| United Way Perth-Huron, Online / Web Service, Connect Youth Perth-Huron | Stratford |
| United Way Perth-Huron, United Centre | Stratford |
| United Way Perth-Huron, Social Research and Planning Council | Stratford |
| United Way Perth-Huron | Stratford |
| United Way Peterborough & District, Backpacks for Success | Peterborough |
| United Way Peterborough & District, Coats for Community | Peterborough |
| United Way Peterborough & District, Administration | Peterborough |
| United Way Simcoe Muskoka, Eat Well to Excel - North Simcoe & South Simcoe | Midland |
| United Way Simcoe Muskoka, Muskoka District, Urgent Needs Fund - Muskoka District | Bracebridge |
| United Way Simcoe Muskoka, Low-Income Energy Assistance Program (LEAP) | Springwater |
| United Way Simcoe Muskoka | Springwater |
| Unity Christian High School | Barrie |
| Universal Workers Union, Barrie Office, Local 183 of Laborers International Union of North America | Barrie |
| University Health Network, Ajmera Transplant Centre, Living Organ Donation Program | Toronto |
| University of Ontario Institute of Technology | Oshawa |
| University of Toronto, Comparative Education Service | Toronto |
| University of Toronto, Faculty of Dentistry, Dental Clinics | Toronto |
| University of Waterloo, Stratford, University of Waterloo Stratford Campus, Stratford Campus | Stratford |
| UPLevel, Stratford, Call Centre | Stratford |
| Uplift Black Centre for Social Justice and Inclusion, Barrie | Barrie |
| Upper Avon River Conservation Club | Perth East |
| Upper Canada Child Care, Alliston - Boyne River Public School, Child Care Centre | New Tecumseth |
| Upper Canada Child Care, Alliston - St Paul's Catholic School, Before and After School Child Care, Alliston - St Paul's Catholic Elementary School | New Tecumseth |
| Upper Canada Child Care, Alliston - Union Public School, Before and After School Child Care, Alliston - Union Elementary School | New Tecumseth |
| Upper Canada Child Care, Alliston - Wellington St E, Child Care Centre | New Tecumseth |
| Upper Canada Child Care, Barrie - Assikinack Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Emma King Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Hyde Park Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Johnson St Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Portage View Public School, Before and After School Child Care, Barrie - Portage View Elementary School | Barrie |
| Upper Canada Child Care, Barrie - St Michael the Archangel Catholic School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Warnica Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Willow Landing Public School, Child Care Centre | Barrie |
| Upper Canada Child Care, Cookstown Public School, Before and After School Child Care | Innisfil |
| Upper Canada Child Care, Everett - Tosorontio Central Public School, Before and After School Child Care | Adjala-Tosorontio |
| Upper Canada Child Care, Innisfil - Lake Simcoe Public School, Before and After School Child Care, Innisfil - Lake Simcoe Elementary School | Innisfil |
| Upper Canada Child Care, Innisfil - Sunnybrae Public School, Before and After School Child Care, Innisfil - Sunnybrae Elementary School | Innisfil |
| Upper Canada Child Care, Oro Station - Guthrie Public School, Before and After School Child Care, Oro Station - Guthrie Elementary School | Oro-Medonte |
| Upper Grand District School Board, Dufferin Centre for Continuing Education, Literacy and Basic Skills, Dufferin Centre for Continuing Education | Orangeville |
| Upper Grand District School Board, Erskine Community Health Centre, Literacy and Basic Skills, Erskine Community Health Centre | Southgate |
| Upper Grand District School Board, Mel Lloyd Centre, Literacy and Basic Skills, Mel Lloyd Centre | Shelburne |
| Upper Grand District School Board, Dufferin Centre for Continuing Education, Adult High School Diploma Program, Dufferin Centre for Continuing Education | Orangeville |
| Upper Thames River Conservation Authority, St. Marys, Wildwood Conservation Area | St Marys |
| Uptown Boxing | Barrie |
| Urban Village Academy, Barrie | Barrie |